产品货号：
GS2187
中文名称：
BstAPI内切酶
英文名称：
BstAPI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity.At 37℃ activiti is 50%from maximum.The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.
技术规格
相关搜索：BstAPI内切酶，BstAPI限制性内切酶
使用说明
High enzyme concentration may result in star activity.At 37℃ activiti is 50%from maximum.The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl. |
| Recognition sites | GCANNNN↑NTGC |
| 来源 | Bacillus stearothermophilus AP |
| Assayed on | Lambda DNA |
| Optimal buffer | W(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 60℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;and 50%glycerol Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 60℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | ApaBI^ |
相关搜索：BstAPI内切酶，BstAPI限制性内切酶